{"id":23325,"date":"2020-07-30T09:00:55","date_gmt":"2020-07-30T01:00:55","guid":{"rendered":"http:\/\/www.gene-optimal.com\/?p=23325"},"modified":"2023-07-19T17:13:54","modified_gmt":"2023-07-19T09:13:54","slug":"mycobacterium-tuberculosis-fluorescent-marker-strain-technical-service","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/mycobacterium-tuberculosis-fluorescent-marker-strain-technical-service\/","title":{"rendered":"\u7ed3\u6838\u5206\u679d\u6746\u83cc\u8367\u5149\u6807\u8bb0\u83cc\u682a"},"content":{"rendered":"[vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”][vc_column_text]\u8367\u5149\u86cb\u767d\u53ef\u4ee5\u7528\u4e8e\u6807\u8bb0\u86cb\u767d\uff0c\u5728\u7279\u5b9a\u6fc0\u53d1\u5149\u6fc0\u53d1\u4e0b\u4f1a\u53d1\u51fa\u7279\u5b9a\u7684\u8367\u5149\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u6d3b\u7ec6\u80de\u5b9e\u65f6\u89c2\u5bdf\u3002\u8367\u5149\u86cb\u767d\u6807\u8bb0\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7279\u5b9a\u86cb\u767d\u540e\uff0c\u53ef\u4ee5\u7814\u7a76\u8be5\u86cb\u767d\u7684\u5b9a\u4f4d\uff0c\u6d4b\u5b9a\u7ed3\u6838\u5206\u679d\u6746\u83cc\u6c27\u5316\u8fd8\u539f\u6c34\u5e73\uff0c\u7814\u7a76\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5728\u52a8\u7269\u7ec6\u80de\u4e2d\u7684\u52a8\u6001\u53d8\u5316\u7b49\uff0c\u6709\u52a9\u4e8e\u7ed3\u6838\u5206\u679d\u6746\u83cc\u514d\u75ab\u5b66\u9886\u57df\u7684\u7814\u7a76\u3002[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”]
\"\"<\/div><\/div>[\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”][vc_column_text]Figure 7. M.tb localizes to LC3-positive compartments. (A) GFPLC3expressing macrophages were infected with mCherry-M.tb (MOI 50<\/p>\n

or 10, 6 h) and then fixed and observed by confocal microscopy. At high MOI there are GFP-LC3 rings surrounding (arrowhead) around mCherry-M.tb and co-localization indicated by yellow pixels (arrow) that are not seen in macrophages challenged at low MOI. (B) LAMP1 immunostaining of GFP-LC3 cell line macrophages infected with M.tb Edrman (MOI 50 or 10, 5 h) demonstrates co-localization of LC3 and lysosomal markers (arrowheads).<\/p>\n

doi:10.1371\/journal.pone.0027972.g007[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”]

\"\"<\/div><\/div>[\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”][vc_column_text]Figure 2. Differences in expression of rv2390c9::GFP in Mtb present in vaccinated versus mock-treated mice. Erdman(rv2390c9::GFP, smyc9::mCherry) was inoculated into vaccinated or mock-treated C57BL\/6J WT mice for up to 56 days. (A) shows 3D confocal images from a 14, 28, or 42 day infection. All bacteria are marked in red (smyc9::mCherry), reporter signal is shown in green (rv2390c9::GFP), nuclei are marked in grayscale (DAPI), and phalloidin staining of f-actin is shown in blue. Scale bar 10 mm. (B) shows quantification of the GFP\/mm3 signal for each bacterium measured from multiple 3D confocal images, at the indicated time points. Each point on the graph represents a bacterium or a tightly clustered group of bacteria (mock-treated \u2013 filled symbols, vaccinated \u2013 open symbols). Horizontal lines mark the median value for each group. p-values were obtained with a Mann-Whitney statistical test.<\/p>\n

doi:10.1371\/journal.ppat.1004394.g002[\/vc_column_text][vc_column_text]\u5e94\u7528\u5206\u679d\u6746\u83cc\u8fc7\u8868\u8fbe\u8d28\u7c92pMV261\uff08\u6e38\u79bb\u578b\uff09\u3001pMV361\uff08\u6574\u5408\u578b\uff09\uff0c\u6784\u5efaeGFP\uff08\u7eff\u8272\u8367\u5149\uff09\u3001mCherry\uff08\u7ea2\u8272\u8367\u5149\uff09\u8367\u5149\u8d28\u7c92\uff0c\u4f7f\u8367\u5149\u86cb\u767d\u5728\u5206\u679d\u6746\u83cc\u5185\u6301\u7eed\u8868\u8fbe\uff0c\u611f\u67d3\u5de8\u566c\u7ec6\u80de\u6216\u5176\u4ed6\u7ec6\u80de\uff0c\u68c0\u6d4b\u611f\u67d3\u6548\u7387\u3001\u7ec6\u80de\u81ea\u566c\u3001\u7ec6\u80de\u51cb\u4ea1\u3001\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5b58\u6d3b\u80fd\u529b\u7b49\u4f5c\u7528\uff1b\u611f\u67d3\u5c0f\u9f20\uff0c\u68c0\u6d4b\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5b9a\u4f4d\uff1b\u53e6\u5916\uff0c\u8367\u5149\u83cc\u682a\u8fd8\u53ef\u7528\u4e8e\u80ba\u5916\u7ed3\u6838\u5982\u80a0\u7ed3\u6838\u3001\u8111\u7ed3\u6838\u3001\u9aa8\u7ed3\u6838\u7b49\u5b9a\u4f4d\u7814\u7a76\uff0c\u52a9\u529b\u4e8e\u7ed3\u6838\u5206\u679d\u6746\u83cc\u81f4\u75c5\u673a\u5236\u7684\u7814\u7a76\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\"\"\u8367\u5149\u83cc\u682a\u793a\u4f8b\u56fe<\/span><\/p>\n

\u6ce8\uff1a\u56feA\u4e3a\u803b\u57a2\u5206\u679d\u6746\u83cc eGFP\u3001mCherry\u8367\u5149\u83cc\u682a\u5728\u84dd\u5149\u6fc0\u53d1\u4e0b\u53d1\u51fa\u7eff\u8272\u548c\u7ea2\u8272\u8367\u5149\uff1b<\/span>\u56feB\u4e3a\u725b\u7ed3\u6838\u5206\u679d\u6746\u83ccBCG eGFP\u3001mCherry\u8367\u5149\u83cc\u682a\u5728\u84dd\u5149\u6fc0\u53d1\u4e0b\u53d1\u51fa\u7eff\u8272\u548c\u7ea2\u8272\u8367\u5149\uff1b<\/span>\u56feC\u4e3a\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra eGFP\u3001mCherry\u8367\u5149\u83cc\u682a\u5728\u84dd\u5149\u6fc0\u53d1\u4e0b\u53d1\u51fa\u7eff\u8272\u548c\u7ea2\u8272\u8367\u5149<\/span><\/p>\n

 <\/p>\n

\u6676\u8bfa\u751f\u7269\u5df2\u6709\u803b\u57a2\u5206\u679d\u6746\u83cc\u3001\u725b\u7ed3\u6838\u5206\u679d\u6746\u83ccBCG\u3001\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Rv\u3001\u7ed3\u6838\u5206\u679d\u6746\u83ccH37Ra\u7684\u7ea2\u8272\u8367\u5149\uff08mCherry\uff09\u3001\u7eff\u8272\u8367\u5149\uff08eGFP\uff09\u83cc\u682a\uff0c\u6b22\u8fce\u5404\u4f4d\u8001\u5e08\u8ba2\u8d2d\u3002<\/span><\/strong>\u6b63\u5728\u7814\u53d1\u84dd\u8272\u8367\u5149\uff08BFP\uff09\u3001\u9ec4\u8272\u8367\u5149\uff08YFP\uff09\u3001\u8fd1\u7ea2\u5916\uff08iRFP720\uff09\u3001\u8367\u5149\u7d20\u9176\u7b49\u6807\u8bb0\u7684\u5206\u679d\u6746\u83cc\u83cc\u682a\uff0c\u8bf7\u5404\u4f4d\u8001\u5e08\u6301\u7eed\u5173\u6ce8\uff01<\/span><\/strong><\/p>\n

 <\/p>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
\u83cc\u682a\u540d\u79f0<\/th>\u89c4\u683c<\/th>\u8fc7\u8868\u8fbe\u8d28\u7c92\u7c7b\u578b<\/th>\u8d27\u53f7<\/th>\n<\/tr>\n<\/thead>\n
MS-pMV261-eGFP\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV261-eGFP<\/td>GOMY0009-2<\/td>\n<\/tr>\n
MS-pMV361-eGFP\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV361-eGFP<\/td>GOMY0009-3<\/td>\n<\/tr>\n
MS-pMV261-mCherry\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV261-mCherry<\/td>GOMY0013-2<\/td>\n<\/tr>\n
MS-pMV361-mCherry\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV361-mCherry<\/td>GOMY0013-3<\/td>\n<\/tr>\n
H37Ra-pMV261-eGFP\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV261-eGFP<\/td>GOMY0010-2<\/td>\n<\/tr>\n
H37Ra-pMV361-eGFP\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV361-eGFP<\/td>GOMY0010-3<\/td>\n<\/tr>\n
H37Ra-pMV261-mCherry\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV261-mCherry<\/td>GOMY0014-2<\/td>\n<\/tr>\n
H37Ra-pMV361-mCherry\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV361-mCherry<\/td>GOMY0014-3<\/td>\n<\/tr>\n
BCG-pMV261-eGFP\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV261-eGFP<\/td>GOMY0011-2<\/td>\n<\/tr>\n
BCG-pMV361-eGFP\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV361-eGFP<\/td>GOMY0011-3<\/td>\n<\/tr>\n
BCG-pMV261-mCherry\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV261-mCherry<\/td>GOMY0015-2<\/td>\n<\/tr>\n
BCG-pMV361-mCherry\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV361-mCherry<\/td>GOMY0015-3<\/td>\n<\/tr>\n
H37Rv-pMV261-eGFP\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV261-eGFP<\/td>GOMY0012-2<\/td>\n<\/tr>\n
H37Rv-pMV361-eGFP\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV361-eGFP<\/td>GOMY0012-3<\/td>\n<\/tr>\n
H37Rv-pMV261-mCherry\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV261-mCherry<\/td>GOMY0016-2<\/td>\n<\/tr>\n
H37Rv-pMV361-mCherry\u8367\u5149\u6807\u8bb0\u83cc\u682a<\/td>1mL\/\u652f<\/td>pMV361-mCherry<\/td>GOMY0016-3<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n

 <\/p>\n\n\n\n\n\t\n\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t\n\t
\u8d28\u7c92\u540d\u79f0<\/th>\u8d27\u53f7<\/th>\n<\/tr>\n<\/thead>\n
pMV261-eGFP\uff0cKan+<\/td>GOPL0017<\/td>\n<\/tr>\n
pMV261-mCherry\uff0cKan+<\/td>GOPL0019<\/td>\n<\/tr>\n
pMV261-iRFP720\uff0cKan+<\/td>GOPL0022<\/td>\n<\/tr>\n
pMV261-eGFP-MCS<\/td>GOPL0017-MCS<\/td>\n<\/tr>\n
pMV261-mCherry-MCS<\/td>GOPL0019-MCS<\/td>\n<\/tr>\n
pMV261\uff0cHyg+\uff08\u6f6e\u9709\u7d20\u6297\u6027\uff09<\/td>GOPL0033<\/td>\n<\/tr>\n
pMV361-eGFP\uff0cKan+<\/td>GOPL0024<\/td>\n<\/tr>\n
pMV361-mCherry\uff0cKan+<\/td>GOPL0026<\/td>\n<\/tr>\n
pMV361-iRFP720\uff0cKan+<\/td>GOPL0028<\/td>\n<\/tr>\n
pMV361-RGP(KN)<\/td>GOPL0035<\/td>\n<\/tr>\n
pMV361-TGP(KN)<\/td>GOPL0036<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n

 <\/p>\n

\u8054\u7cfb\u6211\u4eec<\/strong><\/p>\n

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