{"id":23332,"date":"2018-05-24T01:10:36","date_gmt":"2018-05-23T17:10:36","guid":{"rendered":"http:\/\/www.gene-optimal.com\/?p=23332"},"modified":"2020-07-15T10:51:05","modified_gmt":"2020-07-15T02:51:05","slug":"molecular-biology-technology-services-plasmid-construction","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/molecular-biology-technology-services-plasmid-construction\/","title":{"rendered":"\u5206\u5b50\u751f\u7269\u5b66\u6280\u672f\u670d\u52a1-\u8d28\u7c92\u6784\u5efa"},"content":{"rendered":"[vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”][vc_column_text]\n

\u6280\u672f\u539f\u7406\u4e0e\u5b9e\u9a8c\u6b65\u9aa4<\/strong><\/span><\/h3>\n

\u8d28\u7c92\u662f\u5e7f\u6cdb\u5b58\u5728\u4e8e\u7ec6\u83cc\u3001\u653e\u7ebf\u83cc\u3001\u9175\u6bcd\u83cc\u7b49\u751f\u7269\u4e2d\uff0c\u8d4b\u4e88\u8fd9\u4e9b\u751f\u7269\u6297\u836f\u3001\u6297\u91cd\u91d1\u5c5e\u7b49\u7279\u6027\uff0c\u5bf9\u8d28\u7c92\u8fdb\u884c\u6709\u76ee\u7684\u7684\u6539\u9020\u540e\u53ef\u4ee5\u7528\u4e8e\u76ee\u7684\u57fa\u56e0\u7684\u7247\u6bb5\u6269\u589e\u3001\u76ee\u7684\u57fa\u56e0\u7684\u7279\u5f02\u8868\u8fbe\u7b49\uff0c\u5df2\u7ecf\u6210\u4e3a\u73b0\u4ee3\u751f\u7269\u5b66\u5fc5\u4e0d\u53ef\u5c11\u7684\u5de5\u5177\u3002<\/p>\n

 <\/p>\n

\u8d28\u7c92\u7684\u6784\u5efa\u4e3b\u8981\u7528\u5230\u5e38\u7528\u7684\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u5982PCR\u3001\u9650\u5236\u6027\u6838\u82f7\u9178\u9176\u5207\u3001DNA\u8fde\u63a5\u9176\u8fde\u63a5\u3001\u8f6c\u5316\u5927\u80a0\u6746\u83cc\u611f\u53d7\u6001\u7ec6\u80de\u7b49\u3002PCR\u7528\u6765\u6269\u589e\u76ee\u7684\u57fa\u56e0\u7247\u6bb5\uff0c\u6d89\u53ca\u5f15\u7269\u8bbe\u8ba1\u3001\u9000\u706b\u6e29\u5ea6\u7684\u6478\u7d22\u3001DMSO\u6d53\u5ea6\u4f18\u5316\u7b49\u591a\u79cd\u8fc7\u7a0b\uff0c\u5feb\u901f\u51c6\u786e\u7684PCR\u5bf9\u8d28\u7c92\u6784\u5efa\u8fc7\u7a0b\u6709\u91cd\u8981\u5f71\u54cd\uff0c\u7136\u800c\u6709\u7684\u57fa\u56e0\u7247\u6bb5\u7531\u4e8eGC\u542b\u91cf\u9ad8\u3001\u542b\u6709\u7279\u6b8a\u7ed3\u6784\u7b49\uff0c\u5728\u8fdb\u884cPCR\u65f6\u7279\u522b\u56f0\u96be\uff1b\u8d28\u7c92\u8f7d\u4f53\u6539\u9020\u9700\u8981\u8fdb\u884c\u5404\u79cd\u76ee\u7684\u57fa\u56e0\u7247\u6bb5\u7684\u9176\u5207\u3001\u8fde\u63a5\u7b49\u8fc7\u7a0b\uff0c\u662f\u5f88\u8017\u65f6\u7684\u8fc7\u7a0b\u3002<\/p>\n

 <\/p>\n

\u6676\u8bfa\u751f\u7269\u6709\u4e13\u4e1a\u7684\u7814\u7a76\u4eba\u5458\uff0c\u4ece\u4e8b\u5206\u5b50\u751f\u7269\u5b66\u7814\u7a76\u591a\u5e74\uff0c\u5bf9\u8d28\u7c92\u6784\u5efa\u5bcc\u6709\u7ecf\u9a8c\uff0c\u53ef\u5728\u77ed\u65f6\u95f4\u5185\u9ad8\u6548\u7387\u7684\u5b8c\u6210\u6784\u5efa\u8fc7\u7a0b\u3002\u5728\u4ea7\u54c1\u7814\u53d1\u7684\u8fc7\u7a0b\u4e2d\uff0c\u79ef\u7d2f\u4e86\u4e30\u5bcc\u7684\u8d28\u7c92\u6784\u5efa\u7ecf\u9a8c\uff0c\u5c24\u5176\u64c5\u957f\u7ed3\u6838\u83cc\u3001\u8367\u5149\u4e92\u8865\u3001\u878d\u5408\u86cb\u767d\u7b49\u76f8\u5173\u9886\u57df\u7684\u8d28\u7c92\u6784\u5efa\uff0c\u53ef\u6839\u636e\u987e\u5ba2\u9700\u6c42\u63d0\u4f9b\u76f8\u5173\u6280\u672f\u670d\u52a1\u3002[\/vc_column_text][\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”]

\"\"<\/div><\/div>[\/vc_column][\/vc_row][vc_row type=”in_container” full_screen_row_position=”middle” scene_position=”center” text_color=”dark” text_align=”left” overlay_strength=”0.3″ shape_divider_position=”bottom”][vc_column column_padding=”no-extra-padding” column_padding_position=”all” background_color_opacity=”1″ background_hover_color_opacity=”1″ column_shadow=”none” column_border_radius=”none” width=”1\/1″ tablet_text_alignment=”default” phone_text_alignment=”default” column_border_width=”none” column_border_style=”solid”][vc_column_text]\n

\u7ed3\u6838\u6746\u83cc\u57fa\u56e0<\/strong>PCR<\/strong>\u53ca\u8d28\u7c92\u6784\u5efa\uff1a<\/strong><\/span><\/h3>\n

\u5bf9\u57fa\u56e0\u7ec4\u9ad8GC\u542b\u91cf\u7684\u7ed3\u6838\u83ccPCR\u6709\u72ec\u7279\u7684\u65b9\u6cd5\u548c\u4e30\u5bcc\u7684\u7ecf\u9a8c\uff0c\u53ef\u9ad8\u6548\u7387\u5730\u5b8c\u6210\u9ad8\u96be\u5ea6\u57fa\u56e0PCR\uff0c\u6839\u636e\u5ba2\u6237\u63d0\u4f9b\u7684\u8f7d\u4f53\u4fe1\u606f\uff0c\u9009\u62e9\u5408\u9002\u7684\u9176\u5207\u4f4d\u70b9\uff0c\u7ecf\u9176\u5207\u8fde\u63a5\u540e\u6784\u5efa\u9700\u6c42\u8d28\u7c92\u3002<\/p>\n

 <\/p>\n

\u8367\u5149\u4e92\u8865\u6280\u672f<\/strong>(BiFC)<\/strong>\u8d28\u7c92\u6784\u5efa\uff1a<\/strong><\/span><\/h3>\n

eGFP\u8367\u5149\u86cb\u767d\u5408\u7406\u7684\u62c6\u5206\u5e76\u4e0e\u76ee\u7684\u86cb\u767d\u76f8\u8fde\uff0c\u53ef\u7528\u4e8e\u7814\u7a76\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\uff0c\u6839\u636e\u5ba2\u6237\u9700\u6c42\uff0c\u53ef\u63d0\u4f9b\u8367\u5149\u6807\u8bb0\u8d28\u7c92\u53ca\u7814\u7a76\u86cb\u767d\u76f8\u4e92\u4f5c\u7528\u7684BiFC\u8d28\u7c92\u3002<\/p>\n

 <\/p>\n

\u878d\u5408\u86cb\u767d\u8868\u8fbe\u8d28\u7c92\u6784\u5efa\uff1a<\/strong><\/span><\/h3>\n

\u878d\u5408\u86cb\u767d\u662f\u4e24\u4e2a\u86cb\u767d\u901a\u8fc7\u67d4\u6027\u591a\u80bdlinker\u76f8\u8fde\uff0c\u5177\u6709\u4e24\u4e2a\u86cb\u767d\u7684\u529f\u80fd\uff0c\u5e76\u53ef\u80fd\u5177\u6709\u989d\u5916\u7684\u4f18\u52bf\uff0c\u5982\u6fc0\u6d3b\u65b0\u7684\u4fe1\u53f7\u901a\u8def\u3001\u7279\u5f02\u7684\u7ec6\u80de\u9776\u5411\u6027\u7b49\uff0c\u5728\u65b0\u836f\u7269\u5f00\u53d1\u4e2d\u6709\u5e7f\u9614\u7684\u524d\u666f\uff0c\u6211\u4eec\u5df2\u7ecf\u6784\u5efa\u5e76\u8868\u8fbe\u4e86GMCSF-IL4\u3001IL4-GMCSF\u3001GMCSF-IL10\u3001IL10-GMCSF\u7b49\u591a\u79cd\u878d\u5408\u86cb\u767d\u8d28\u7c92\uff0c\u7ecf\u9a8c\u4e30\u5bcc\uff0c\u6839\u636e\u5ba2\u6237\u63d0\u4f9b\u7684\u57fa\u56e0\u3001\u8f7d\u4f53\u4fe1\u606f\uff0c\u6211\u4eec\u53ef\u63d0\u4f9b\u9700\u6c42\u878d\u5408\u86cb\u767d\u8868\u8fbe\u8d28\u7c92\u3002<\/p>\n

 <\/p>\n

\u670d\u52a1\u6d41\u7a0b\uff1a<\/strong><\/span><\/h3>\n
    \n
  1. \u5ba2\u6237\u63d0\u4f9b\u76ee\u7684\u57fa\u56e0\u53ca\u8f7d\u4f53\u4fe1\u606f\u53ca\u9700\u6c42<\/li>\n
  2. \u8bbe\u8ba1\u5f15\u7269\uff0c\u8fdb\u884cPCR\uff0cPCR\u4ea7\u7269\u3001\u8f7d\u4f53\u8fdb\u884c\u9176\u5207\u5e76\u8fde\u63a5\u8f6c\u5316\uff0c\u6311\u53d6\u83cc\u843d\u63d0\u53d6\u8d28\u7c92\u6d4b\u5e8f<\/li>\n
  3. \u53d1\u9001\u4ea7\u54c1\uff1a\u6784\u5efa\u597d\u7684\u9633\u6027\u83cc\u6db2\u53ca\u8d28\u7c92\uff0c\u76f8\u5173\u7684\u5f15\u7269\u4fe1\u606f\uff0cPCR\u3001\u9176\u5207\u7535\u6cf3\u56fe\uff0c\u6d4b\u5e8f\u7ed3\u679c\u3002<\/li>\n<\/ol>\n

    \u670d\u52a1\u5468\u671f\uff1a1-2\u5468[\/vc_column_text][\/vc_column][\/vc_row]\n","protected":false},"excerpt":{"rendered":"

    [vc_row type=”in_co…<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[5],"tags":[],"_links":{"self":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/23332"}],"collection":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/comments?post=23332"}],"version-history":[{"count":1,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/23332\/revisions"}],"predecessor-version":[{"id":40866,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/23332\/revisions\/40866"}],"wp:attachment":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media?parent=23332"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/categories?post=23332"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/tags?post=23332"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}