{"id":32373,"date":"2018-07-27T15:38:39","date_gmt":"2018-07-27T07:38:39","guid":{"rendered":"http:\/\/www.gene-optimal.com\/?p=32373"},"modified":"2018-07-27T15:38:39","modified_gmt":"2018-07-27T07:38:39","slug":"protease","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/protease\/","title":{"rendered":"\u86cb\u767d\u9176\u7c7b"},"content":{"rendered":"

\u86cb\u767d\u9176\uff08Protease\uff09\u662f\u6c34\u89e3\u86cb\u767d\u8d28\u80bd\u94fe\u7684\u4e00\u7c7b\u9176\u7684\u603b\u79f0\u3002\u86cb\u767d\u8868\u8fbe\u7eaf\u5316\u65f6\u5e38\u7528HRv3C\uff08HRv3C\u86cb\u767d\u9176\uff09\u3001Ulp1\uff08SUMO\u86cb\u767d\u9176\uff09\u3001rTEV\uff08\u91cd\u7ec4\u70df\u8349\u75c5\u6bd2\u86cb\u767d\u9176\uff09\u7b49\u86cb\u767d\u9176\u5207\u9664\u86cb\u767d\u6807\u7b7e\u3002<\/p>\n

 <\/p>\n

HRv3C\uff08HRv3C\u86cb\u767d\u9176\uff09<\/p>\n

\u7ecf\u8fc7\u57fa\u56e0\u5de5\u7a0b\u6539\u9020\u540e\u7684\u91cd\u7ec4\u86cb\u767d\u9176<\/p>\n

\u7279\u5f02\u6027\u8bc6\u522b\uff1aLeu-Glu-Val-Leu-Phe-Gln-Gly-Pro\u516b\u6c28\u57fa\u9178\u5e8f\u5217<\/p>\n

\u526a\u5207\u4f4d\u70b9\uff1a\u5728Gln-Gly\u4e4b\u95f4<\/p>\n

\u542b\u6807\u7b7e\uff1aGST\u6807\u7b7e\u3001His\u6807\u7b7e<\/p>\n

\u4f5c\u7528\u540e\u53bb\u9664\u65b9\u6cd5\uff1aGSH\u6811\u8102\u6216Ni\u67f1<\/p>\n

 <\/p>\n

Ulp1\uff08SUMO\u86cb\u767d\u9176\uff09<\/p>\n

\u5177\u6709\u8f83\u9ad8\u6d3b\u6027\u7684\u534a\u80f1\u6c28\u9178\u86cb\u767d\u9176<\/p>\n

\u7279\u5f02\u6027\u8bc6\u522b\uff1aSUMO\u86cb\u767d\u7684\u4e09\u7ea7\u7ed3\u6784<\/p>\n

\u542b\u6807\u7b7e\uff1a\u591a\u805aHis\u6807\u7b7e<\/p>\n

\u4f5c\u7528\u540e\u53bb\u9664\u65b9\u6cd5\uff1a\u4eb2\u548c\u5c42\u6790<\/p>\n

\u7279\u6027\uff1a\u53ef\u4ee5\u9ad8\u6548\u800c\u4e14\u7279\u5f02\u6027\u5730\u5c06SUMO\u86cb\u767d\u4ece\u91cd\u7ec4\u878d\u5408\u86cb\u767d\u4e0a\u5207\u5272\u4e0b\u6765\uff0c\u5728\u8f83\u5bbd\u8303\u56f4\u7684\u53cd\u5e94\u73af\u5883\u4f53\u7cfb\u4e2d\u80fd\u4fdd\u6301\u8f83\u9ad8\u7684\u6d3b\u6027\uff0c\u5982\u6e29\u5ea6\uff084-30\u2103\uff09\uff0cPH (5.5-9.5)\u7b49<\/p>\n

 <\/p>\n

rTEV\uff08\u91cd\u7ec4\u70df\u8349\u75c5\u6bd2\u86cb\u767d\u9176\uff09<\/p>\n

\u7ecf\u8fc7\u57fa\u56e0\u5de5\u7a0b\u6539\u9020\u540e\u7684\u91cd\u7ec4\u86cb\u767d\u9176<\/p>\n

\u7279\u5f02\u6027\u8bc6\u522b\uff1aGlu-Asn-Leu-Tyr-Phe- Gln-Gly \u4e03\u6c28\u57fa\u9178\u5e8f\u5217<\/p>\n

\u542b\u6807\u7b7e\uff1a\u8be5\u9176\u7ecf 6\u00d7His \u6807\u7b7e\u7eaf\u5316\u800c\u5f97\uff08\u542b\u7ec4\u80fa\u9178\u6807\u7b7e\uff09\uff0c\u7eaf\u5ea6\u8fbe99\uff05<\/p>\n

\u4f5c\u7528\u540e\u53bb\u9664\u65b9\u6cd5\uff1aHis\u6807\u7b7e\u7eaf\u5316\u6811\u8102Ni-NTA Resin(Cat.No. P2010)<\/p>\n

\u7279\u6027\uff1arTEV \u86cb\u767d\u9176\u4e0e\u80a0\u6fc0\u9176 U(EK)\u3001Thrombin\u3001FactorXa\u3001SUMO \u7b49\u86cb\u767d\u9176\u76f8\u6bd4\uff0c\u5177\u6709\u9ad8\u6d3b\u6027\u3001\u9ad8\u7279\u5f02\u6027\u7684\u53cc\u91cd\u7279\u70b9\uff0crTEV \u86cb\u767d\u9176\u56e0\u5177\u9ad8\u526a\u5207\u6d3b\u6027\u548c\u7279\u5f02\u6027\uff0c\u5df2\u6210\u4e3a\u878d\u5408\u86cb\u767d\u8868\u8fbe\u540e\u53bb\u9664\u878d\u5408 tag \u7684\u9996\u9009\u86cb\u767d\u9176\u3002<\/p>\n

 <\/p>\n

\"\"<\/p>\n

TEV\u86cb\u767d\u9176\u7ed3\u6784<\/span><\/p>\n

 <\/p>\n

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\u86cb\u767d\u9176\uff08Protease\uff09\u662f\u6c34\u89e3\u86cb\u767d\u8d28\u80bd\u94fe\u7684\u4e00\u7c7b\u9176…<\/p>\n","protected":false},"author":1,"featured_media":32349,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[93],"tags":[],"_links":{"self":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/32373"}],"collection":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/comments?post=32373"}],"version-history":[{"count":2,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/32373\/revisions"}],"predecessor-version":[{"id":32376,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/posts\/32373\/revisions\/32376"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media\/32349"}],"wp:attachment":[{"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/media?parent=32373"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/categories?post=32373"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.gene-optimal.com\/wp-json\/wp\/v2\/tags?post=32373"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}