{"id":41426,"date":"2024-01-05T08:00:46","date_gmt":"2024-01-05T00:00:46","guid":{"rendered":"https:\/\/www.gene-optimal.com\/?p=41426"},"modified":"2024-01-05T10:00:42","modified_gmt":"2024-01-05T02:00:42","slug":"tb-wgs","status":"publish","type":"post","link":"https:\/\/www.gene-optimal.com\/tb-wgs\/","title":{"rendered":"\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f"},"content":{"rendered":"<p><span style=\"color: #3366ff;\"><strong><span style=\"font-size: 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src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx03.png\" alt=\"\" width=\"1399\" height=\"362\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx03.png 1399w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx03-150x39.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx03-300x78.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx03-768x199.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx03-1024x265.png 1024w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx03-200x52.png 200w\" sizes=\"(max-width: 1399px) 100vw, 1399px\" 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RFLP\u3001spoligotype\u3001VNTR\u7b49\u57fa\u56e0\u5206\u578b\u65b9\u6cd5\u9274\u5b9a\u7ed3\u6838\u75c5\u4f20\u64ad\u94fe\uff0c\u4f46\u4f7f\u7528\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u7684\u65b9\u6cd5\u76f8\u8f83\u4e8e\u57fa\u56e0\u5206\u578b\u5177\u6709\u66f4\u591a\u7684\u4f18\u52bf\uff0c\u5982\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u5177\u6709\u66f4\u9ad8\u7684\u5206\u8fa8\u7387\uff0c\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u662f\u57fa\u4e8e\u57fa\u56e0\u7ec4\u95f4\u7684SNP\u5dee\u5f02\u6570\u6765\u786e\u5b9a\u4e24\u4e24\u83cc\u682a\u95f4\u662f\u5426\u5177\u6709\u8fd1\u671f\u4f20\u64ad\u5173\u7cfb\uff0c\u800c\u4f20\u7edf\u57fa\u56e0\u5206\u578b\u53ea\u662f\u57fa\u4e8e\u90e8\u5206\u57fa\u56e0\u8fdb\u884c\u533a\u5206\uff0c\u7ed3\u679c\u53ef\u80fd\u9020\u6210\u57fa\u56e0\u578b\u76f8\u540c\u7684\u83cc\u682a\u5b58\u5728\u4e0d\u540c\u7684\u4f20\u64ad\u6e90\u548c\u4f20\u64ad\u8def\u5f84\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u4f20\u7edf\u57fa\u56e0\u5206\u578b\u5f88\u96be\u51c6\u786e\u9274\u522b\u4f20\u64ad\u53d1\u751f\u7684\u8def\u5f84\u3001\u987a\u5e8f\u53ca\u662f\u5426\u5b58\u5728\u591a\u4e2a\u4f20\u64ad\u6e90\uff0c\u800c\u901a\u8fc7\u5168\u57fa\u56e0\u7ec4\u6570\u636e\u5206\u6790\u53ef\u89e3\u51b3\u6b64\u7c7b\u95ee\u9898\u3002<\/p>\n<p>\u9057\u4f20\u8ddd\u79bb\u5206\u6790\u793a\u4f8b\uff1a<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42764\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx04.2.png\" alt=\"\" width=\"925\" height=\"434\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx04.2.png 925w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx04.2-150x70.png 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx04.2-300x141.png 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx04.2-768x360.png 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx04.2-200x94.png 200w\" sizes=\"(max-width: 925px) 100vw, 925px\" \/><br \/>\n\u8be5\u56fe\u4e3a\u6bcf\u4e24\u4e2a\u83cc\u682a\u95f4\u7684SNP\u5dee\u5f02\u6570\u77e9\u9635\uff0c\u4e00\u822c\u8ba4\u4e3aSNP<12\u4e2a\uff0c\u4e24\u83cc\u682a\u95f4\u5177\u6709\u8fd1\u671f\u4f20\u64ad\u5173\u7cfb\uff0c\u53ef\u7ed3\u5408\u6d41\u884c\u75c5\u5b66\u8c03\u67e5\u9274\u5b9a\u5730\u533a\u7ed3\u6838\u75c5\u7684\u4f20\u64ad\u94fe\u3002<\/p>\n<p>\u6b64\u9879\u5206\u6790\u5728\u6d41\u884c\u75c5\u5b66\u8c03\u67e5\u7814\u7a76\u4e2d\u66f4\u5177\u6709\u660e\u663e\u4f18\u52bf\uff0c\u968f\u7740\u9ad8\u901a\u91cf\u6d4b\u5e8f\u7684\u666e\u904d\u5e94\u7528\u53ca\u6210\u672c\u4f18\u52bf\uff0c\u6709\u671b\u53d6\u4ee3\u4f20\u7edf\u7684\u57fa\u56e0\u5206\u578b\u65b9\u6cd5\uff0c\u6210\u4e3a\u5206\u5b50\u6d41\u884c\u75c5\u5b66\u7684\u91d1\u6807\u51c6\u3002<\/p>\n<p>\u9664\u4e86\u4ee5\u4e0a\u5e38\u89c4\u5206\u6790\u5185\u5bb9\uff0c\u6676\u8bfa\u65b0\u589e\u4e2a\u6027\u5316\u5206\u6790\u5185\u5bb9\u2014\u2014\u6784\u5efa\u7cfb\u7edf\u53d1\u80b2\u6811\uff0c\u5e76\u5c06\u4ee5\u4e0a\u7684\u6570\u636e\u5185\u5bb9\u8fdb\u884c\u6c47\u603b\u56fe\u5f62\u5316\u663e\u793a\uff0c\u66f4\u6e05\u6670\u533a\u5206\u5404\u83cc\u682a\u95f4\u5173\u7cfb\u3001\u836f\u7269\u7684\u654f\u611f\u6027\u3001\u8c31\u7cfb\u53ca\u8010\u836f\u5206\u7c7b\u3002<\/p>\n<p>\u7cfb\u7edf\u8fdb\u5316\u6811\u793a\u4f8b\uff1a<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-42765\" src=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx05.jpg\" alt=\"\" width=\"1200\" height=\"679\" srcset=\"https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx05.jpg 1200w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx05-150x85.jpg 150w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx05-300x170.jpg 300w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx05-768x435.jpg 768w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx05-1024x579.jpg 1024w, https:\/\/www.gene-optimal.com\/wp-content\/uploads\/2019\/05\/cx05-200x113.jpg 200w\" sizes=\"(max-width: 1200px) 100vw, 1200px\" \/><br \/>\n\u56fe\u4e2d\u7ebf\u957f\u77ed\u7528\u4e8e\u8868\u793a\u4e24\u4e2a\u83cc\u682a\u95f4\u7684\u9057\u4f20\u8ddd\u79bb\uff0c\u8c31\u7cfb\u3001\u8010\u836f\u5206\u7c7b\u4f7f\u7528\u989c\u8272\u8fdb\u884c\u4e86\u533a\u5206\uff0c\u836f\u7269\u654f\u611f\u6027\u4f7f\u7528\u4e86\u7a7a\u5b9e\u5708\u8fdb\u884c\u6807\u6ce8\uff0c\u9ed1\u8272\u5706\u5708\u8868\u793a\u8010\u53d7\u5bf9\u5e94\u836f\u7269\uff0c\u767d\u8272\u5706\u5708\u8868\u793a\u5bf9\u5e94\u836f\u7269\u654f\u611f\u3002<\/p>\n<p>\u9664\u6b64\u4e4b\u5916\uff0c\u4e5f\u53ef\u5b9a\u5236\u5176\u5b83\u9ad8\u7ea7\u5206\u6790\u5185\u5bb9\uff0c\u6b22\u8fce\u54a8\u8be2\uff01<\/p>\n<p>&nbsp;<\/p>\n<p><span style=\"font-size: 18pt; color: #3366ff;\"><strong>\u5206\u679d\u6746\u83cc\u57fa\u56e0\u7ec4\u63d0\u53d6\u6837\u54c1\u5236\u5907\u65b9\u6cd5<\/strong><\/span><\/p>\n<p><strong>\u4e00\uff0e\u6837\u54c1\u57f9\u517b<\/strong><\/p>\n<p><strong>1.\u56fa\u4f53\u57f9\u517b<\/strong><\/p>\n<p>\u5438\u53d6\u4e00\u5b9a\u4f53\u79ef\u7684\u83cc\u6db2\uff08\u6839\u636e\u83cc\u4f53\u6d53\u5ea6\u8c03\u6574\u5438\u53d6\u4f53\u79ef\uff09\u81f3\u56fa\u4f53\u57f9\u517b\u57fa\u5212\u7ebf\uff08\u5355\u7ebf\u6cd5\u3001\u56db\u533a\u6cd5\u5747\u53ef\uff09\uff0c\u9521\u7b94\u7eb8\u5305\u88f9\uff0c37\u00b0C\u5012\u7f6e\u57f9\u517b\uff0c\u76f4\u81f3\u514b\u9686\u76f4\u5f84\u4e3a0.5-1 cm\u5927\u5c0f\u3002<\/p>\n<p><strong>2.\u6db2\u4f53\u57f9\u517b<\/strong><\/p>\n<p>\u6311\u53d6\u5355\u514b\u9686\u6216\u5438\u53d6\u4e00\u5b9a\u4f53\u79ef\u7684\u83cc\u6db2\uff08\u6839\u636e\u83cc\u4f53\u6d53\u5ea6\u8c03\u6574\u5438\u53d6\u4f53\u79ef\uff09\u81f3\u6db2\u4f53\u57f9\u517b\u57fa\u4e2d\uff0c\u57f9\u517b\u57fa\u4f53\u79ef\u4e0d\u8d85\u8fc7\u5bb9\u5668\u603b\u4f53\u79ef\u76841\/5\u6216\u7279\u6b8a\u5bb9\u5668\u89c4\u5b9a\u7684\u6700\u5927\u57f9\u517b\u4f53\u79ef\u300237\u00b0C\u9759\u7f6e\u57f9\u517b\uff0c\u76f4\u81f3\u83cc\u6db2\u6d53\u5ea6\u8fbe\u5230OD600=0.8-1\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><strong>\u4e8c. \u6837\u54c1\u706d\u6d3b<\/strong><\/p>\n<p><strong>1.\u83cc\u843d\u5904\u7406\u65b9\u6cd5<\/strong><\/p>\n<p>\u6311\u53d6\u9ec4\u8c46\u7c92\u5927\u5c0f\u7684\u83cc\u843d\u81f3TE\u6eb6\u6db2\uff0825 mM Tris-HCl (pH 8.0)\uff0c10 mM EDTA\uff09\u4e2d\uff0c\u5c3d\u91cf\u907f\u514d\u6311\u53d6\u4efb\u4f55\u56fa\u4f53\u57f9\u517b\u57fa\uff08\u6df7\u6709\u57f9\u517b\u57fa\u4f1a\u5f71\u54cd\u540e\u7eed\u57fa\u56e0\u7ec4\u63d0\u53d6\u6548\u679c\uff09\uff0c<strong>TE\u6eb6\u6db2\u4f53\u79ef\u9700\u6df9\u6ca1\u83cc\u843d<\/strong>\u3002\u91d1\u5c5e\u6d7480\u2103\u52a0\u70ed30\u5206\u949f\u3002\u706d\u6d3b\u540e\u7684\u6837\u54c1\u7acb\u5373\u53d6\u51fa\u5e76\u51bb\u5b58\u4e8e-80\u2103\u51b0\u7bb1\u3002\u5bc4\u9001\u524d\u8bf7\u52ff\u53cd\u590d\u51bb\u878d\u3002<\/p>\n<p><strong>2.\u83cc\u6db2\u5904\u7406\u65b9\u6cd5<\/strong><\/p>\n<p>\u5438\u53d61mL\u6d53\u5ea6\u8fbe\u5230OD600=0.8-1\u65b0\u9c9c\u83cc\u6db2\u81f3\u87ba\u7eb9\u7ba1\u4e2d\uff0c13000 rpm\u79bb\u5fc310\u5206\u949f\u3002\u5f03\u6389\u4e0a\u6e05\uff0c\u52a0\u5165500 \u03bcL\u7684TE\u6eb6\u6db2\uff0825 mM Tris-HCl (pH 8.0)\uff0c10 mM EDTA\uff09\u3002\u91d1\u5c5e\u6d7480\u2103\u52a0\u70ed30\u5206\u949f\u3002\u706d\u6d3b\u540e\u7684\u6837\u54c1\u7acb\u5373\u53d6\u51fa\u5e76\u51bb\u5b58\u4e8e-80\u2103\u51b0\u7bb1\u3002\u5bc4\u9001\u524d\u8bf7\u52ff\u53cd\u590d\u51bb\u878d\u3002<\/p>\n<table width=\"604\">\n<tbody>\n<tr>\n<td width=\"121\">\u83cc\u682a\u5206\u7c7b<\/td>\n<td width=\"61\">\u5e8f\u53f7<\/td>\n<td width=\"180\">\u83cc\u682a\u540d\u79f0<\/td>\n<td width=\"242\">\u706d\u6d3b\u6761\u4ef6<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"20\" width=\"121\">\u7ec6\u83cc<\/td>\n<td width=\"61\">1<\/td>\n<td width=\"180\">\u6c99\u95e8\u6c0f\u83cc<\/td>\n<td width=\"242\">80\u2103\u6c34\u6d7415min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">2<\/td>\n<td width=\"180\">\u5fd7\u8d3a\u6c0f\u83cc<\/td>\n<td width=\"242\">70\u2103\u6c34\u6d7415min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">3<\/td>\n<td width=\"180\">\u5355\u6838\u7ec6\u80de\u589e\u751f\u674e\u65af\u7279\u6c0f\u83cc<\/td>\n<td width=\"242\">80\u2103\u6c34\u6d7415min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">4<\/td>\n<td width=\"180\">\u526f\u6eb6\u8840\u6027\u5f27\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">5<\/td>\n<td width=\"180\">\u7ed3\u6838\u5206\u679d\u6746\u83cc<\/td>\n<td width=\"242\">80\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">6<\/td>\n<td width=\"180\">\u5510\u83d6\u84b2\u4f2f\u514b\u970d\u5c14\u5fb7<\/td>\n<td width=\"242\">80\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">7<\/td>\n<td width=\"180\">\u80ba\u708e\u94fe\u7403\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">8<\/td>\n<td width=\"180\">\u6d41\u611f\u55dc\u8840\u6746\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">9<\/td>\n<td width=\"180\">\u970d\u4e71\u5f27\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">10<\/td>\n<td width=\"180\">\u521b\u4f24\u5f27\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">11<\/td>\n<td width=\"180\">\u975e\u7ed3\u6838\u5206\u679d\u6746\u83cc<\/td>\n<td width=\"242\">80\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">12<\/td>\n<td width=\"180\">\u5927\u80a0\u57c3\u5e0c\u6c0f\u83cc<\/td>\n<td width=\"242\">70\u2103\u6c34\u6d7415min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">13<\/td>\n<td width=\"180\">\u9c8d\u66fc\u4e0d\u52a8\u6746\u83cc<\/td>\n<td width=\"242\">80\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">14<\/td>\n<td width=\"180\">\u5e7d\u95e8\u87ba\u6746\u83cc<\/td>\n<td width=\"242\">80\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">15<\/td>\n<td width=\"180\">\u80ba\u708e\u514b\u96f7\u4f2f\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">16<\/td>\n<td width=\"180\">\u652f\u539f\u4f53<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">17<\/td>\n<td width=\"180\">\u94dc\u7eff\u5047\u5355\u80de\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7460min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">18<\/td>\n<td width=\"180\">\u6c99\u95e8\u6c0f\u83cc<\/td>\n<td width=\"242\">80\u2103\u6c34\u6d7415min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">19<\/td>\n<td width=\"180\">\u91d1\u9ec4\u8272\u8461\u8404\u7403\u83cc<\/td>\n<td width=\"242\">80\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">20<\/td>\n<td width=\"180\">\u5316\u8113\u94fe\u7403\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7430min<\/td>\n<\/tr>\n<tr>\n<td rowspan=\"3\" width=\"121\">\u771f\u83cc<\/td>\n<td width=\"61\">1<\/td>\n<td width=\"180\">\u767d\u5047\u4e1d\u9175\u6bcd\u83cc(\u767d\u8272\u5ff5\u73e0\u83cc)<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7460min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">2<\/td>\n<td width=\"180\">\u9a6c\u5c14\u5c3c\u83f2\u7bee\u72b6\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7460min<\/td>\n<\/tr>\n<tr>\n<td width=\"61\">3<\/td>\n<td width=\"180\">\u65b0\u751f\u9690\u7403\u83cc<\/td>\n<td width=\"242\">60\u2103\u6c34\u6d7410min<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u4ee5\u4e0a\u5747\u4e3a\u6700\u4f73\u706d\u6d3b\u6761\u4ef6\uff0c\u5982\u6e29\u5ea6\u8fc7\u9ad8\u3001\u65f6\u95f4\u8fc7\u957f\uff0c\u6216\u6ca1\u6709\u52a0TE buffer\uff0c\u5219\u53ef\u80fd\u5f71\u54cd\u63d0\u53d6\u6548\u679c\u3002<\/p>\n<p><span style=\"color: #0000ff;\"><strong>\u8bf7\u52ff\u5c06\u5e72\u83cc\u4f53\u76f4\u63a5\u8fdb\u884c\u706d\u6d3b\uff0c\u6839\u636e\u6676\u8bfa\u5b9e\u9a8c\u5ba4\u7684\u64cd\u4f5c\u6807\u51c6\u6d41\u7a0b\uff0c\u5e72\u706d\u7684\u6837\u672c\u4f1a\u88ab\u8ba4\u4e3a\u4ecd\u7136\u5b58\u5728\u98ce\u9669\uff0c\u5982\u4ecd\u9700\u7ee7\u7eed\u9879\u76ee\uff0c\u6211\u4eec\u5c06\u542f\u52a8\u751f\u7269\u5b89\u5168\u5b9e\u9a8c\u5ba4\u6d41\u7a0b\u5e76\u4ea7\u751f\u8d39\u7528\uff0c\u8bf7\u6ce8\u610f\uff01<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p>\u5982\u9700TE Buffer\uff0c\u6211\u4eec\u6709100 mL\u548c500 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\u2461\u2026\u2026\u7b49\u7279\u6b8a\u5b57\u7b26\uff0c\u5982\u6709\uff0c\u5206\u6790\u65f6\u6211\u4eec\u4f1a\u7528\u62fc\u97f3\u66ff\u6362\u4e2d\u6587\uff0c\u6570\u5b57\u66ff\u6362\u7279\u6b8a\u5b57\u7b26\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>\u5206\u4eab\u4e00\u4e9b\u6709\u5173\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u5206\u65b9\u9762\u7684\u7efc\u8ff0\u6587\u7ae0\uff0c\u5e0c\u671b\u80fd\u5bf9\u7ed3\u6838\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u611f\u5174\u8da3\u7684\u60a8\u6709\u6240\u5e2e\u52a9\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u6587\u732e\u5206\u4eab\uff1a<\/p>\n<p>1\u3001\u5f20\u6d01,\u4efb\u6021\u5ba3,\u6f58\u4e3d\u840d,\u5f20\u5b97\u5fb7.\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f\u5728\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7814\u7a76\u4e2d\u7684\u5e94\u7528[J].\u4e2d\u56fd\u9632\u75e8\u6742\u5fd7\uff0c2020\u5e747\u6708\u7b2c42\u5377\u7b2c7\u671f.<\/p>\n<p>\u94fe\u63a5\uff1ahttp:\/\/www.zgflzz.cn\/CN\/10.3969\/j.issn.1000-6621.2020.07.017<\/p>\n<p>2\u3001\u5f90\u9e4f,\u7518\u660e\u5b87,\u9ad8\u8c26.\u4e8c\u4ee3\u6d4b\u5e8f\u6280\u672f\u5728\u7ed3\u6838\u5206\u679d\u6746\u83cc\u7814\u7a76\u4e2d\u7684\u5e94\u7528\u8fdb\u5c55[J].\u5fae\u751f\u7269\u4e0e\u611f\u67d3\uff0c2015\u5e742\u670825\u65e5\uff0c10\uff081\uff09\uff1a54-60.<\/p>\n<p>\u94fe\u63a5\uff1ahttp:\/\/jmi.fudan.edu.cn\/CN\/abstract\/abstract491.shtml<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7ed3\u6838\u5206\u679d\u6746\u83cc\u5168\u57fa\u56e0\u7ec4\u6d4b\u5e8f 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